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Planta Med 1980; 40(10): 137-148
DOI: 10.1055/s-2008-1074950
Research Articles

© Georg Thieme Verlag Stuttgart · New York

Alkaloids and Lipids of Heterotrophic, Photomixotrophic and Photoautotrophic Cell Suspension Cultures of Peganum harmala1

Wolfgang Barz1 , Hildegard Herzbeck1 , Wolfgang Hüsemann1 , Georg Schneiders2 , Helmut K. Mangold2
  • 1Institute of Plant Biochemistry, University of Münster,
  • 2Institute for Biochemistry and Technology - H. P. Kaufmann-Institute - Federal Center of Lipid Research, Münster, Federal Republic of Germany
1 Dedicated to Prof. Dr. Drs. h.c. KURT MOTHES on the occasion of his 80th birthday
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Publication History

Publication Date:
29 April 2008 (online)

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Abstract

Heterotrophic, photomixotrophic and photoautotrophic cell suspension cultures of Peganum harmala were analysed for harman alkaloids which could only be found in heterotrophic cultures. When photoautotrophic cell cultures were reverted to heterotrophic cultures, harman alkaloids and serotonin were again found and accumulated in the cultures. The alkaloid pattern obtained depended on the phytohormones applied. The lipids in photoautotrophic cell cultures were found to contain high proportions of monogalactosyldiacylglycerols and digalactosyldiacylglycerols. These lipids of photoautotrophic cultures contained higher proportions of constituent linolenic acid, but lower concentrations of linoleic acid than those of heterotrophic cultures. The fatty acid patterns of individual lipid classes and the sterol fractions of the 3 types of cell cultures were compared and significant differences are discussed.

Key Word Index

Peganum harmala - Photoautotrophic Cell Cultures - Harman Alkaloids - Serotonin - Lipids - Sterols.